Tropical Journal of Pharmaceutical Research

Original Research Article | OPEN ACCESS

Protective effect of salvianolic acid B against intestinal ischemia reperfusion-induced injury in a rat model

Jiaxi Chen¹ , Weiming Zhou², Zhibin Zhou³, Tangzhan Yuan¹, Bo Li¹, Yang Zheng¹

¹Department of General Surgery, The Fourth Affiliated Hospital of Nanchang University, NanChang, Jiangxi 330000; ²Department of Vascular Surgery, The Second Affiliated Hospital of Nanchang University, NanChang, Jiangxi 330006; ³Department of Research, The Fourth Affiliated Hospital of Nanchang University, NanChang, Jiangxi 330000,.

For correspondence:- Jiaxi Chen Email: jaixichen@hotmail.com Tel:+8679186663001

Accepted: 23 September 2017 Published: 31 October 2017

Citation: Chen J, Zhou W, Zhou Z, Yuan T, Li B, Zheng Y. Protective effect of salvianolic acid B against intestinal ischemia reperfusion-induced injury in a rat model. Trop J Pharm Res 2017; 16(10):2431-2438 doi: 10.4314/tjpr.v16i10.17

© 2017 The authors.
This is an Open Access article that uses a funding model which does not charge readers or their institutions for access and distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0) and the Budapest Open Access Initiative (http://www.budapestopenaccessinitiative.org/read), which permit unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited..

Abstract

Purpose: To evaluate the gastro-protective efficacy of salvianolic acid B (SAB) against intestinal ischemic-reperfusion injury (IIRI) in a rat model.

Methods: Forty-eight healthy male rats were randomly choosen and divided into 4 groups of 12 rats each. Control group rats underwent laparotomy without occlusion; IIRI group rats underwent laparotomy with occlusion for 60 min, followed by 24 h of reperfusion; SAB + IIRI group received 7 days of pretreatment with 40 mg/kg of SAB + IIRI; while the fourth group received only SAB. The antioxidant, inflammatory markers, intestinal permeability marker, as well as intestinal histopathological changes were assessed.

Results: The activities of antioxidants including reduced glutathione (GSH), catalase (CAT) and superoxide dismutase (SOD) were significantly ameliorated (p < 0.01) in SAB-supplemented group (SAB + IIRI). The concentration of inflammatory markers, including interleukin-1β (IL-1β), interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-α) and nuclear factor p65 (NF-p65) as well as small intestinal permeability marker (FITC-Dextran), were significantly reduced (p < 0.01) following administration of SAB for 7 days. In addition, pretreatment with SAB reverted intestinal (ileum) histopathological changes to almost normal architecture with significant reduction in Chiu score.

Conclusion: The results of this study demonstrate that SAB may protect the intestine by attenuating oxidative stress and inflammatory response and hence, may be potentially for treating IIRI.

Keywords: Salvianolic acid B, Intestinal Ischemia-reperfusion, Antioxidants, Inflammation, Intestinal permeability